Biofilm Removal In-vitro study In-vitro biofilm removal from human enamel using a Philips Sonicare Power Flosser
B. Gottenbos; W. Suntjens; S. Hötzl Philips Research Europe, 2019
Quantify removal of in-vitro dental biofilm using the Philips Sonicare Power Flosser oral irrigator.
Dental biofilms were grown from pooled human saliva on human enamel disks for 4 days, according to an established academic model*. The biofilms (n=6) were treated with the Philips Sonicare Power Flosser for 3 seconds using the Quad Stream nozzle. To quantify the number of bacteria before treatment, the biofilm volume was measured using optical coherence tomography (OCT) and the bacterial cell density was determined from untreated control samples (n=6) using confocal laser scanning microscopy (CLSM). After treatment the number of remaining bacteria were counted using CLSM. Additionally scanning electron microscope (SEM) images were recorded.
While before treatment 0.2 mm thick dense biofilms were present, after treatment only scattered groups of bacteria remained. Quantitative analysis showed 99.96% removal for the Quad Stream nozzle.
The Philips Sonicare Power Flosser oral irrigator with Quad Stream nozzle removes over 99.9% of the bacteria in this established laboratory model of dental biofilm.
OCT cross-sections: no visible biofilm remains after treatment.
SEM images at 2500x magnification.
* Exterkate RA, Crielaard W, Ten Cate JM. Di erent response to amine uoride by Streptococcus mutans and polymicrobial biofilms in a novel high-throughput active attachment model. Caries Res. 2010;44(4):372-9.
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